Learn How To Do Seaman Test ?

SEMEN ANALYSIS

The Semen test is the primary test for infertility diagnosis. In this test, the following information of seaman is noted۔

Volume	مقدار	1
pH	پی اچ	2
Percantage of Motile Spermatozoa	حرکت میں موجود سپرم کی مقدار	3
Sperm Count	سپرم کی ٹوٹل کاؤنٹ	4
Morphology of Sperm	سپرم کی اشکال	5

SAMPLE OF SEMEN

Why is semen test needed?

If there is no child for a while after marriage, then a semen test is done After male vasectomy, a semen test is done to see the complications of the operation

Normal state of semen:

Normal semen laces
And sticky.
It has a special smell
Its normal volume ranges from 1.5-5 ml
Its deficiency affects the sperm count

Getting money

Laboratory tests that require a semen sample have a specific method of obtaining the sample Before obtaining a semen sample, the man must not have intercourse with the woman for one week and must not have ejaculated in any other way.

The best way to achieve this is to ejaculate and collect the semen in a clean, sterile vial, but if a man cannot masturbate or does not like this method, The solution is for him to have intercourse with his wife and as soon as he feels ejaculation, pull out the penis and collect the semen in a vial. 

If this is not possible, then keep it in a clean bottle at home and within thirty minutes it should be brought to the laboratory and the laboratory staff should be told how much time has passed since the semen sample was taken. Using condoms to collect semen is the wrong way. Samples for   

The patient is instructed:

That within 15-30 minutes the intra sample will be delivered to the laboratory .Samples for semen tests are best obtained in the laboratory Sexual intercourse should be avoided for three days before giving. 

:Special Precautions

The laboratory worker should process the semen sample with extreme care as it may contain the following pathogen

Hepatitis B, C

HIV

Syphilis

Gonorrhea

Chlaymdia

Herpes

:Medical examination

A few similar things are seen in the medical examination

  The quantity of semen is observed. Normal quantity ranges from 1.5 to 5 and the sample quantity report is prepared accordingly. The pH of the semen is monitored Normal pH ranges from eight to nine 

 

 VOLUM OF SEMEN

.

Semen is in a condensed semi-solid state after it is excreted from the body.

Its state changes to liquid form in about an hour.

When the cement turns into a liquid state, the quantity can be determined in any marked tube

NORMAL VOLUME

TWO 

  Should be more than milliliters

Don't know PH

pH paper is used to determine pH. Put a drop of semen on the page paper with a pipette۔A normal Ph is 7.2 within an hour

 

Percntage of Motile & Viable Spermatoza

Count of motile sperm:

  (Motility)

Place a drop of liquid cement on a clean glass slide and cover with a cover slip.
On Power then see (40). Focus the sample in the (10x) microscope first
Count 100 sperm while focusing in the microscope and see how many are motile and how many are not.. Normal movement (within an hour)
should be more than (50%)

Vaible or Non vaible Sperm

Live or dead sper

Put one drop of liquid semen (10-15) on a glass slide and add one drop of Eosin, mix and place the slide. (40x) and then (10x) after two minutes focus the slide in the microscope. First Live sperm do not stain while dead sperm do.  Find the number of live and dead sperm out of one hundred sperm. Normal live/dead count (within one hour): More than 75% of sperm are alive

 (Total Count of Sperm)سپرم کی ٹوٹل کاؤنٹ

Pipette one milliliter of semen into a clean, dry, labeled small tube.

Then add 20 ml of sodium bicarbonate formalin and mix well.This Simmons sample will be made with a ratio of 1:20 to total.

Keep this mixture for five minutes. Focus and fill it with the Ge seman mixer above. Neubar chamber in the microscope Wait five minutes after filling the chamber and focus the sepal at 10x.And find the amount of sperm in the two large round boxes.

In two boxes its volume is 2sq mm.

Procedure

 (Calculations)فارمولا

Sperm in1 ml  =         NO    Of Sperm

          

                           * 100000

Counted in 2 sq mm

نارمل کاونٹ 

یا اس سے زیادہ ہو سکتی ہے۔(10x20)     

         Sperm Morphology

 Procedure

Spread a thin layer of cement on a glass slide and allow it to air dry

and mix in v/v 99% ethanol and allow to dry.Then wash the fixed layer with sodium bicarbonate formalin solution, 

Then dip the slide in clean water 4-5 times.Put carbon fuxin 1:20 on the slide and leave it for 3 minutes then wash it off.Put low fur methylene blue as a counterstain on the slide and wash after two minutes.Focus the slide under the microscope and observe the shapes of the sperm۔

(Staining)سپرم کی سٹین

Nucleaus of Head------ Dark Blue

Cytoplasm of Head------ Pale Blue

Middle Piece & Tail------ Pink Red

Count the different shapes in 100 sperm and separate normal and abnormal among them.

Normal Sperm Morphology:

Normal sperm are 50-70 micrometers in length. The head is oval in shape with a cap of Acre Visom mounted on it. The head is followed by the middle part and its long dome.50% of normal value Or should look more normal

Abnormal Sperm Morphology:  

The following forms may be seen
Sperm size too small or too large.
They were the size of the bed and the head.
Not having a layer of Acre Wasom.
The middle picea is missing or the size is too large. Crooked midsection.
The center component is split into parts.
Absence of the tail (tail) splitting into 2 parts or becoming crooked
Does the nucleus contain vacuoles or chromatin?

:Terminology

.

Aspermia  means that there is no discharge of semen from the penis
Oligospermia means low sperm count
Hypospermia  means that the emission of semen is low
Azoospermia means that there is no sperm at all
Oligozoospermia means the count is less than 20 million.
Polyzoospermia means that the sperm count is more than 350 million.
Asthenozoospermia means that sperm do not move or move very slowly.
Oligoasthenzoospermia means that the number of sperm is low and it is also very weak in movement.

THANK YOU

Next