Lab Equipment and uses | Lab Instruments and There Use ?



 Lab Instruments and There Use

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Centrifuge:

Use to partition of plasma/serum from end lessly blood parts. 
Cleaning/partition of organic blend test for 
example DNA, RNA, Proteins, RBC, WBC, Pus Cells

Centrifuge

The germs or particles in the liquid are collected at the bottom of the test tube by a centrifuge machine.  The higher the speed of rotation, the less weight the particles accumulate in the layer. The following two types of centrifuge machines are used in the laboratory. 

1). For general purpose 

2). Hematocrit centrifuge.

Microscope

Use to magnify or enlarge micro
specimen to study structure, size
and Shape of cellular particles
.

Definition:

It is an instrument which produces enlarged images of

minute objects.

1 Different body parts of microscope.

1. Ocular lens, or eyepiece

2. Objective turret

3. Objective lens

4. Coarse adjustment knob

5. Fine adjustment knob

6. Object holder or stage

7. Mirror or light (illuminator)

8. Diaphragm and condenser

9. Mechanical stage

Types Of Microscope:

1. Electron Microscope.

2. Light Microscope 

(It can be simple or compound).

A). Simple microscope:

It is a simple magnifying hand lens. Its magnification

power is 2x to 200x.

B). Compound microscope:

This has a battery of lens, which are fitted in a

complex instrument. It can be monocular or binocular.

1. Monocular has single eye piece.

2. Binocular has 2 eye pieces.

Parts of a Compound Microscope:

1. STAND gives stability.

2. BODY  consists of.

1) Body tubes.

2) Stage

3)Knobs

1) BODY TUBES: 

There are two tubes.

- External tube: carries objective lens.

- Internal Tube: carries ocular lens.

2) STAGE:

glass slide.

It is a metallic platform which accommodates

It has an aperture in its center which permits the light to

reach the object. Just below the stage is a substage which

consists of condenser, through which light is focused on object.

3) KNOBS

Knobs are present on either side of the body "Coarse

"Adjustment" feel like bigger knobs, movement of which moves were body tabs with its lens. "Fine adjustment"


The senses were smaller knobs on the ether side of the body.

Has  smaller knobs on both aspect of the body.

3. OPTICAL SYSTEM:

Consists of eye pieces, objectives and condensers.

EYE PIECE:

The magnification of an eye piece can be 5x, 10x, 15x.

- OBJECTIVES:

The magnification of objectives can be 4x, 10x, 40x, 100x.

- CONDENSER:

This is made up of two simple lens and it condenses light on the object.

Hot Air Oven

Hot air ovens are electrical devices
which is use dry heat to sterilize of glassware,
Cotton Swab, Culture media. they can be
operated from 50 to 300 °C

Autoclave

Use to Sterilize (Removing or killing of bacteria) 
glassware, chemical, culture media
At 121'C, 15lbs pressure for 20 minutes.

instructions:

Sterilization of the following items is done by laboratory autoclave Sterilizable items used in the laboratory.  Samples left over from tests in the laboratory / sample culture media after use before use.

Essential precautions for using an autoclave.  

Add water to the autoclave according to the manufacturer's instructions. Place the items in the autoclave in such a way that steam can easily pass through them, and do not touch each other or touch the wall of the autoclave.  Follow the manufacturer's instructions. Close the lid. Set the auto-cleave time and pressure. After achieving the specified time and pressure, turn off the auto-cleave and wait for it to cool.

PCR Machine 

Polymerase Chain Reaction (PCR)

(PCR machine or DNA (amplifier) 
is a laboratory apparatus most commonly used
to amplify segments of DNA via the
polymerase chain reaction (PCR).

Gel Electrophoresis

Use to Separation or identification of
DNA, RNA, Proteins.

Digital PH Meter


Use to measure
or set a constant
PH Value

Hot Plant with Magnetic Stirrer


Hot plate use to Heat Solution.
Magnetic stirrer use to blending an answer on a
combined hot-plate

Spectrophotometer

Spectrophotometers measure light 
intensity as a function of wavelength
and are commonly used to measure the
concentration of a compound in an aqueous
solution..


B.O.D. Incubator


Refrigerated Incubators often called 

B.O.D. or Low Temperature Incubators) are
commonly used for applications such as B.O.D.
(Biological Oxygen Demand) Determinations,
Plant and Insect Studies, Fermentation Studies,
Bacterial Culturing and Mycology Studies


 Laminar Air Flow

Use to sterilize specific working
environment while pour culture plate.

Table of Contents:

Water Bath

Yat is asad to incobate simples in water at a
constant temperature (20-60'c) over a long
period of time

Water bath is used for the following. 

For bottle/flask of culture media, for test tubes and culture samples etc.  Uses and Special Precautions for Water Bath  Read and follow the manufacturer's instructions carefully.  Keep the amount of water in the water bath as per the manufacturer's instructions. 


Always use distilled water in the water bath and change according to the manufacturer's instructions and never add any chemicals.  Check the temperature before placing the sample in the water bath.  Place items in the waterbath for the recommended temperature and time.  Clean the water tank according to the manufacturer's 

Incubator

Incubator is a tool used to develop and
maintain microbiological cultures or cell cultures.
The incubator maintain.s optimal temperature, humidity

History of the laboratory incubator

From helping in hatching bird eggs to allowing scientists to apprehend and increase vaccines for lethal viruses, the laboratory incubator has visible several packages through the years it's been in use. The incubator has additionally furnished a basis for clinical advances and experimental paintings in mobile and molecular biology.



While many technological advances have happened for the reason that primitive incubators were first utilized in historical Egypt and China, the principle motive of the incubator has remained unchanged: to create a stable, managed surroundings conducive to research, study, and cultivation.






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