What is Polymerase Chain Reaction(P.C.R)

Polymerase Chain Reaction(P.C.R)

PCR is a modern diagnostic test, thanks to which many diseases, especially hepatitis, TB, etc., can be diagnosed quickly and easily. It is possible in the early stages. PCR stands for polymerase chain reaction. It is a sensitive test thanks to which any cell The DNA in me is artificially converted into thousands of such duplicates or templates and then By finding out its quantity, the presence or absence of the disease is confirmed.

For example:

The DNA present in the hepatitis virus is amplified with the help of Amplimers. These are small single-stranded DNA molecules that are the DNA of the diseased cell bind themselves with These DNA molecules also bind an enzyme called DNA A. Molly Maries says that under certain conditions a special machine called a thermal cycler (thermal cycler) can multiply DNA twenty times.

It does. Which in general terms means that a single DNA molecule is made up of a one million copies.which means that even if the DNA of the virus is in a very small amount, It increases it to ten million times and thus it becomes very easy to prove the presence of the virus. Thanks to this method, one On the one hand, the presence of the virus is known, while on the other hand, the exact number of the virus is also known. Number of viruses (Copies) 

Even after the treatment, it is seen again that the patient has become free from viruses so that further treatment is needed or not. In this way, the different types of viruses are also known as genotypes and the specific habits of each genotype. The duration of that treatment is fixed.

This method of treatment is becoming popular nowadays in the diagnosis of various diseases. Including hepatitis B&C and TB etc. are included.

This test is 99% - 96% sensitive. (Specific)

This test is 98% - 95% sensitive.

This test is 99.9% reliable. (Reliable)

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